Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa x Populus deltoides) phenylalanine ammonia-lyase genes.
Identifieur interne : 004B67 ( Main/Exploration ); précédent : 004B66; suivant : 004B68Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa x Populus deltoides) phenylalanine ammonia-lyase genes.
Auteurs : R. Subramaniam [Canada] ; S. Reinold ; E K Molitor ; C J DouglasSource :
- Plant physiology [ 0032-0889 ] ; 1993.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Arbres (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Développement des plantes (MeSH), Famille multigénique (MeSH), Gènes de plante (MeSH), Hybridation génétique (MeSH), Phenylalanine ammonia-lyase (génétique), Plantes (enzymologie), Plantes (génétique), Régulation de l'expression des gènes (MeSH), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- enzymologie : Plantes.
- génétique : ADN complémentaire, Phenylalanine ammonia-lyase, Plantes.
- Arbres, Clonage moléculaire, Données de séquences moléculaires, Développement des plantes, Famille multigénique, Gènes de plante, Hybridation génétique, Régulation de l'expression des gènes, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (genetics), Gene Expression Regulation (MeSH), Genes, Plant (MeSH), Hybridization, Genetic (MeSH), Molecular Sequence Data (MeSH), Multigene Family (MeSH), Phenylalanine Ammonia-Lyase (genetics), Plant Development (MeSH), Plants (enzymology), Plants (genetics), Sequence Homology, Amino Acid (MeSH), Trees (MeSH).
- MESH :
- chemical , genetics : DNA, Complementary, Phenylalanine Ammonia-Lyase.
- enzymology : Plants.
- genetics : Plants.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Genes, Plant, Hybridization, Genetic, Molecular Sequence Data, Multigene Family, Plant Development, Sequence Homology, Amino Acid, Trees.
Abstract
A heterologous probe encoding phenylalanine ammonia-lyase (PAL) was used to identify PAL clones in cDNA libraries made with RNA from young leaf tissue of two Populus deltoides x P. trichocarpa F1 hybrid clones. Sequence analysis of a 2.4-kb cDNA confirmed its identity as a full-length PAl clone. The predicted amino acid sequence is conserved in comparison with that of PAL genes from several other plants. Southern blot analysis of popular genomic DNA from parental and hybrid individuals, restriction site polymorphism in PAL cDNA clones, and sequence heterogeneity in the 3' ends of several cDNA clones suggested that PAL is encoded by at least two genes that can be distinguished by HindIII restriction site polymorphisms. Clones containing each type of PAL gene were isolated from a poplar genomic library. Analysis of the segregation of PAL-specific HindIII restriction fragment-length polymorphisms demonstrated the existence of two independently segregating PAL loci, one of which was mapped to a linkage group of the poplar genetic map. Developmentally regulated PAL expression in poplar was analyzed using RNA blots. Highest expression was observed in young stems, apical buds, and young leaves. Expression was lower in older stems and undetectable in mature leaves. Cellular localization of PAL expression by in situ hybridization showed very high levels of expression in subepidermal cells of leaves early during leaf development. In stems and petioles, expression was associated with subepidermal cells and vascular tissues.
DOI: 10.1104/pp.102.1.71
PubMed: 8108506
PubMed Central: PMC158748
Affiliations:
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Reinold</name></author><author><name sortKey="Molitor, E K" sort="Molitor, E K" uniqKey="Molitor E" first="E K" last="Molitor">E K Molitor</name></author><author><name sortKey="Douglas, C J" sort="Douglas, C J" uniqKey="Douglas C" first="C J" last="Douglas">C J Douglas</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8108506</idno><idno type="pmid">8108506</idno><idno type="pmc">PMC158748</idno><idno type="doi">10.1104/pp.102.1.71</idno><idno type="wicri:Area/Main/Corpus">004B74</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004B74</idno><idno type="wicri:Area/Main/Curation">004B74</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004B74</idno><idno type="wicri:Area/Main/Exploration">004B74</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa x Populus deltoides) phenylalanine ammonia-lyase genes.</title><author><name sortKey="Subramaniam, R" sort="Subramaniam, R" uniqKey="Subramaniam R" first="R" last="Subramaniam">R. Subramaniam</name><affiliation wicri:level="4"><nlm:affiliation>Department of Botany, University of British Columbia, Vancouver, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Botany, University of British Columbia, Vancouver</wicri:regionArea><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique</region></placeName><orgName type="university">Université de la Colombie-Britannique</orgName></affiliation></author><author><name sortKey="Reinold, S" sort="Reinold, S" uniqKey="Reinold S" first="S" last="Reinold">S. Reinold</name></author><author><name sortKey="Molitor, E K" sort="Molitor, E K" uniqKey="Molitor E" first="E K" last="Molitor">E K Molitor</name></author><author><name sortKey="Douglas, C J" sort="Douglas, C J" uniqKey="Douglas C" first="C J" last="Douglas">C J Douglas</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>Gene Expression Regulation (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Hybridization, Genetic (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Multigene Family (MeSH)</term><term>Phenylalanine Ammonia-Lyase (genetics)</term><term>Plant Development (MeSH)</term><term>Plants (enzymology)</term><term>Plants (genetics)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Arbres (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Développement des plantes (MeSH)</term><term>Famille multigénique (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Hybridation génétique (MeSH)</term><term>Phenylalanine ammonia-lyase (génétique)</term><term>Plantes (enzymologie)</term><term>Plantes (génétique)</term><term>Régulation de l'expression des gènes (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Phenylalanine Ammonia-Lyase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Plantes</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Phenylalanine ammonia-lyase</term><term>Plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Gene Expression Regulation</term><term>Genes, Plant</term><term>Hybridization, Genetic</term><term>Molecular Sequence Data</term><term>Multigene Family</term><term>Plant Development</term><term>Sequence Homology, Amino Acid</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Développement des plantes</term><term>Famille multigénique</term><term>Gènes de plante</term><term>Hybridation génétique</term><term>Régulation de l'expression des gènes</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A heterologous probe encoding phenylalanine ammonia-lyase (PAL) was used to identify PAL clones in cDNA libraries made with RNA from young leaf tissue of two Populus deltoides x P. trichocarpa F1 hybrid clones. Sequence analysis of a 2.4-kb cDNA confirmed its identity as a full-length PAl clone. The predicted amino acid sequence is conserved in comparison with that of PAL genes from several other plants. Southern blot analysis of popular genomic DNA from parental and hybrid individuals, restriction site polymorphism in PAL cDNA clones, and sequence heterogeneity in the 3' ends of several cDNA clones suggested that PAL is encoded by at least two genes that can be distinguished by HindIII restriction site polymorphisms. Clones containing each type of PAL gene were isolated from a poplar genomic library. Analysis of the segregation of PAL-specific HindIII restriction fragment-length polymorphisms demonstrated the existence of two independently segregating PAL loci, one of which was mapped to a linkage group of the poplar genetic map. Developmentally regulated PAL expression in poplar was analyzed using RNA blots. Highest expression was observed in young stems, apical buds, and young leaves. Expression was lower in older stems and undetectable in mature leaves. Cellular localization of PAL expression by in situ hybridization showed very high levels of expression in subepidermal cells of leaves early during leaf development. In stems and petioles, expression was associated with subepidermal cells and vascular tissues.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8108506</PMID><DateCompleted><Year>1994</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>102</Volume><Issue>1</Issue><PubDate><Year>1993</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Structure, inheritance, and expression of hybrid poplar (Populus trichocarpa x Populus deltoides) phenylalanine ammonia-lyase genes.</ArticleTitle><Pagination><MedlinePgn>71-83</MedlinePgn></Pagination><Abstract><AbstractText>A heterologous probe encoding phenylalanine ammonia-lyase (PAL) was used to identify PAL clones in cDNA libraries made with RNA from young leaf tissue of two Populus deltoides x P. trichocarpa F1 hybrid clones. Sequence analysis of a 2.4-kb cDNA confirmed its identity as a full-length PAl clone. The predicted amino acid sequence is conserved in comparison with that of PAL genes from several other plants. Southern blot analysis of popular genomic DNA from parental and hybrid individuals, restriction site polymorphism in PAL cDNA clones, and sequence heterogeneity in the 3' ends of several cDNA clones suggested that PAL is encoded by at least two genes that can be distinguished by HindIII restriction site polymorphisms. Clones containing each type of PAL gene were isolated from a poplar genomic library. Analysis of the segregation of PAL-specific HindIII restriction fragment-length polymorphisms demonstrated the existence of two independently segregating PAL loci, one of which was mapped to a linkage group of the poplar genetic map. Developmentally regulated PAL expression in poplar was analyzed using RNA blots. Highest expression was observed in young stems, apical buds, and young leaves. Expression was lower in older stems and undetectable in mature leaves. Cellular localization of PAL expression by in situ hybridization showed very high levels of expression in subepidermal cells of leaves early during leaf development. 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sort="Douglas, C J" uniqKey="Douglas C" first="C J" last="Douglas">C J Douglas</name><name sortKey="Molitor, E K" sort="Molitor, E K" uniqKey="Molitor E" first="E K" last="Molitor">E K Molitor</name><name sortKey="Reinold, S" sort="Reinold, S" uniqKey="Reinold S" first="S" last="Reinold">S. Reinold</name></noCountry><country name="Canada"><region name="Colombie-Britannique"><name sortKey="Subramaniam, R" sort="Subramaniam, R" uniqKey="Subramaniam R" first="R" last="Subramaniam">R. Subramaniam</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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